Fig. 3

DIXDC1 knockdown in EC result in reduced angiogenic potential. A BrdU immunostaining of EC treated with either control siRNA or DIXDC1 siRNA (60 nM). Scale bars 50 μm. B MTT assay of EC treated with control siRNA or DIXDC1 siRNA in basal and VEGF-A-treated condition (percentage of control). C Transwell migration assay of EC after silencing of DIXDC in EC. Migration of EC was significantly decreased when DIXDC1 is silenced in both basal and VEGF-A-treated condition (40 ng/ml). D Quantification of transwell migration assay. E Ability of tube-like structure formation of EC decreased when expression of DIXDC1 was suppressed in EC in both basal and presence of VEGF-A. F, G, and H Quantification of tube formation assay (Percentage of control). I Sprouting of EC was decreased when DIXDC1 expression was knocked down in EC. J, K Quantification of sprouting assay. All experiments were repeated at least 5 different sets of ECs treated with siRNA or DIXDC1 siRNA. *p < 0.05, **p < 0.005, and ***p < 0.0001, by one-way ANOVA and 2-tailed Student’s t test. Error bars represent the mean ± SD. Individual values can be found in Additional file 6: Fig. 3