Fig. 7
DIXDC1-KO mice displayed decreased neovascularization. A Hemorrhage in WT and Dixdc1 knockout mice retinae after exposure to high oxygen level (75% O2) for 5 days. (OIR model) n = 6 per group. B Whole-mount CD31 immunostaining of retinae from A. Scale bars 500 μm. C, D Quantification of relative avascular region and neovascularization. E In vivo wound healing assay of 8-week-old WT and DIXDC1-KO mice. n = 6 per group. F Quantification of wound healing assay. G H&E staining of horizontal section of wound area of WT and DIXDC1-KO mice. Scale bar 50 μm. H CD31 and VEGFR2 immunostaining of horizontal section of wounded skin of WT and Dixdc1 KO mice. I, J Quantification of relative CD31 and VEGFR2 level. All Experiments were repeated at least 3 different sets of WT and DIXDC1-KO littermates. *p < 0.05, **p < 0.005, and ***p < 0.0001, by paired, 2-tailed Student’s t test. Error bars represent the mean ± SD. Individual values can be found in Additional file 6: Fig. 7