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Fig. 3 | BMC Biology

Fig. 3

From: Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics

Fig. 3

Heterologous localization of candidate MRO proteins of Pelomyxa schiedti. C-terminally GFP-tagged proteins were expressed in Saccharomyces cerevisiae. Mitochondria were stained with MitoTracker. Note that gene sequences were amplified from cDNA using specific primers (Additional file 1: Table S4), except for P5CR that was synthesized in vitro. ACL, ATP-citrate lyase; cpn10, chaperonin 10; DIC, differential interference contrast; FdhD, formate dehydrogenase accessory sulfurtransferase; HydA, [FeFe]-hydrogenase; NifS, cysteine desulfurase; NifU, scaffold protein; P5CR, pyrroline-5-carboxylate reductase. Scale bar, 5 μm

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BMC Biology

ISSN: 1741-7007

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