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Fig. 4 | BMC Biology

Fig. 4

From: BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos

Fig. 4

NANOG- and GATA6-double-negative cells are emerged from JQ1-treated blastocysts and appear to re-induce NANOG-positive cells reversibly (stages 3 to 4). A–D, B’, B”, B”’, D’, D”, D”’ NANOG- and GATA6-doube-negative cells emerged after JQ1 treatment (yellow arrows). Immunohistochemical analysis of NANOG (magenta) and GATA6 (green), with DAPI (nuclei, blue) staining in control (A, B, B’, B”, B”’) and 5 μM JQ1-treated blastocysts (C, D, D’, D”, D”’) for 6 h at E3.5. E Schematic illustration of the experimental strategy, with or without JQ1, for an additional 24-h culture. FK, G’, G”, G”’, I’, I”, I”’, K’, K”, K”’ NANOG- and GATA6-double-negative cells are re-specified into NANOG-positive cells after JQ1 removal. Immunohistochemical analysis of NANOG (magenta) and GATA6 (green), with DAPI (blue) staining, in mouse blastocysts at E3.5 treated without JQ1 for 30 h (F, G, G’, G”, G”’), with 5 μM JQ1 for 30 h (H, I, I’, I”, I”’), and with 5 μM JQ1 for 6 h and without JQ1 for 24 h (J, K, K’, K”, K”’). The sample numbers analyzed for each experiment are indicated in Additional file 19. Scale bars, 20 μm in B, B’, B”, B”’, D, D’, D”, D”’, G, G’, G”, G”’, I, I’, I”, I”’, K, K’, K”, K”’; 40 μm in A, C, F, H, and J. DMSO, dimethyl sulfoxide

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