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Fig. 6 | BMC Biology

Fig. 6

From: BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos

Fig. 6

The epiblast lineage can be specified in Brd4-deficient blastocysts (stage 3). A, A’ Immunohistochemical analysis of BRD4 protein (magenta; A), and DAPI (nuclei, blue; A’) staining, in the Brd4lacZ/lacZ embryos at E3.5. The expression of BRD4 protein is undetectable in a Brd4lacZ/lacZ embryo. B, C Immunohistochemical analysis of NANOG (magenta), with DAPI (blue) staining, in wild type (WT) (B) or Brd4lacZ/lacZ E3.5 blastocysts (C). NANOG expression was not reduced in a Brd4lacZ/lacZ blastocyst. D The ratio of numbers of NANOG-expressing cells to DAPI-positive cells (nuclei) in WT and Brd4lacZ/lacZ E3.5 blastocysts (two-tailed Mann-Whitney’s U-test; n.s., not significant; p = 0.571). Red lines indicate the mean values, and green lines represent the SE bars. E–N Whole-mount in situ hybridization analysis in WT (E–I) and Brd4lacZ/lacZ E3.5 blastocysts (J–N). The expression of Nanog (E, J), Sox2 (F, K), Gata6 (G, L), Oct3/4 (H, M), or Cdx2 (I, N) mRNAs. Individual values of marker-expressing cells are provided in Additional file 15. The sample numbers analyzed for each experiment are indicated in Additional file 19. Scale bars, 25 μm in E–N; 40 μm in A–C, A’

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