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Fig. 6 | BMC Biology

Fig. 6

From: Verticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence

Fig. 6

Functional differentiation of the CFEM-containing VdSCPs in Verticillium dahliae. A Virulence assays with CFEM-containing VdSCPs gene deletion mutant strains of V. dahliae on cotton. Three-week-old cotton seedlings were inoculated with wild-type V. dahliae and gene deletion mutant strains, and the disease symptoms were observed at 3 weeks after inoculation. B Botrytis cinerea infection assays in the 4-week-old Nicotiana benthamiana leaves that were firstly transient expression with Agrobacterium tumefaciens Gv3101 carrying the vector harboring GFP as control on the left of leaves and carrying the recombinant vector harboring CFEM-containing VdSCPs on the right of leaves. The phenotypes (B) and diameters (C) of lesions were examined at 4 days after inoculation with B. cinerea. Error bars represent standard errors. ** and *** represent statistical significance at P < 0.01 and P < 0.001, respectively, between the different VdSCPs and GFP control according to one-way analysis of variance (ANOVA)

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