Fig. 2

Effect of shift work on the cycling heart transcriptome. A Total number (left) and overlap (right) between rhythmically expressed genes for each group: control (C, black), shift worker (W, blue), or shift worker subjected to restricted feeding during work (WRF, red). B Heatmap visualization of rhythmic gene expression for genes rhythmic in both C and WRF groups, C only, W only, and WRF only. The signal for each timepoint corresponds to the average of 3 individual samples. Genes were ordered based on the phase of rhythmic gene expression of C rats for the top two heatmaps, while they were ordered based on the phase of rhythmic gene expression in W and WRF groups for genes rhythmic in W only and WRF only, respectively. C Rose plot representation of the phase of rhythmic gene expression in C, W, and WRF rat groups. D Clock gene rhythmic expression in the heart of C, W, and WRF rats. The signal corresponds to the mean +/- s.e.m of 3 animals per timepoint. Statistical analysis for rhythmic gene expression is displayed as R (rhythmic expression) or A (arrhythmic expression), and the font color refers to each group: C, black; W, blue; WRF, red. E KEGG pathway enrichment for genes being rhythmically expressed in C, W, and WRF rat heart. Pathways that are not enriched in certain groups (p > 0.05) are displayed in grey. Diameter of each circle is also proportional to the enrichment q value, with higher diameter for lower q values. F Expression profile of genes accounting for the enrichment of three KEGG pathways. The expression of individual genes is displayed in gray and the averaged expression in red. For the oxidative phosphorylation and endocytosis pathways (WRF enriched pathways), the rhythmic expression of one gene is in antiphase to all other genes; this gene is displayed in orange and was not taken into account for calculating the averaged expression