Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: Identification and characterisation of spontaneous mutations causing deafness from a targeted knockout programme

Fig. 4

The rhme deletion spanning Map3k5 and Map7 causes progressive hearing loss and disrupts the organ of Corti. a Schematic of the rhme deletion seen in the MEEK line showing the different isoforms of the two affected genes (not to scale). Red indicates missing exons. b Mean ABR thresholds showing progressive hearing loss in mice homozygous for the deletion (wildtype shown by black inverted triangles, heterozygote by blue circles, homozygotes by red triangles) (n = 1 wildtype, 6 heterozygotes and 12 homozygotes at P28-32; 1 wildtype, 19 heterozygotes and 11 homozygotes at P42-57; 1 wildtype, 5 heterozygotes and 6 homozygotes at P63-84 (see Additional File 1: Fig. S9a for individual thresholds)). Traces from individual homozygotes are shown in grey; error bars on mean trace are standard deviations. c Expression of MYO7A, a hair cell marker, in the organ of Corti of adult mice showing an apical turn (top; 94% of the distance along the organ of Corti from base to apex), where the hair cells are still present in affected mice, and a mid-basal turn (bottom, 43% of the distance from base to apex), where only the inner hair cell is identifiable (hair cells are marked with arrowheads; red for inner hair cells, black for outer hair cells). Brown indicates where MYO7A is expressed. Three affected and 3 unaffected littermates (at matched ages between 33 and 84 days old) were examined, and the images shown are representative of our observations. Scale bar = 50 µm. Data underlying plots in this figure are in Additional File 3

Back to article page