Fig. 6
Effect of external phase reference marker on phase relationship in baboon and human blood transcriptome. For Per3 in baboon bladder and human blood, the z-scored expression profiles are plotted against time since a the start of light, b the midpoint of light period and c end of light, for the baboon in purple and for human blood in red (mean ± SD for interpolated data points and just mean for double-plotted data points) and actual data points for each participant are shown in grey (n = 19). In each panel, the strength of circular cross-correlation between the alignment of the baboon expression profile and the human diurnal expression profile is listed (xcor), together with the lag in hours between the expression profiles. The relative light/dark cycles for baboon (purple) and human diurnal (red) conditions are indicated as horizontal bars below the plots (white = light, black = dark). Lags were calculated for all overlapping rhythmic genes in every baboon tissue/organ based on alignment with start of light, midpoint of light and end of light. The resulting three lags per gene, per baboon tissue, were ranked such that the alignment with the smallest lag is given the top rank (1). d Heatmap and e boxplot of averaged ranked lags of all overlapping rhythmic genes, where ranking was performed within gene across the three different alignments and average was calculated for all genes within a baboon tissue/organ (n = 64)