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Fig. 6 | BMC Biology

Fig. 6

From: Epigenetic regulation of seed-specific gene expression by DNA methylation valleys in castor bean

Fig. 6

Functional analysis of distal DMVs. A Average enrichment of histone modifications over distal DMVs (black lines) and all DMVs (blue lines) in the endosperm. The read count of the ChIP-seq signal for each histone modification was averaged over DMVs and the upstream and downstream 2-kb regions. B Example of a distal DMV and its nearest gene YABBY1 (YAB1, upper panel) and landscape of different histone modifications in leaves and endosperm (lower panel). C Schematic representation of vectors used for functional validation of the YAB1-distal DMV using the dual-luciferase reporter assay system in Nicotiana benthamiana protoplasts. D Relative luciferase activity, as determined by the ratio of firefly LUC to REN luciferase activity. Error bars indicated SDs from five biological replicates. Statistical significance was determined by a two-sided Student’s t-test

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