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Fig. 2 | BMC Biology

Fig. 2

From: A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver

Fig. 2

In vivo amiR-based gene silencing in the mouse liver. a Brightfield and fluorescence stereomicroscopic images of the liver of Fah−/− mice 5 months after the intrahepatic delivery of an amiR-free control and different amiR-expressing transposon vectors. b Monitoring the amount of the endogenous p53 mRNA and artificial transcripts A and B in the liver of Fah−/− mice 5 months after intrahepatic delivery of an amiR-free control and different amiR-expressing transposon vectors. Liver RNA samples were collected from Fah−/− mice at 5 months post-treatment. Samples were tested using Fah-, EGFP-, and p53 mRNA-specific RT-qPCR assays. Results were normalized to measurements of the ribosomal protein L27 (Rpl27) transcript as input control and data were presented as the mean ± SD (n = 3) (see Additional file 2 for individual data values and statistics). c Monitoring of endogenous p53 mRNA levels in NIH3T3 cells after stable transposon-based delivery of different amiR elements designed to silence Tp53 expression. RNA samples were collected from cultured cells after G418 selection and tested using a p53 mRNA-specific RT-qPCR assay. Results were normalized to measurements of the ribosomal protein L27 (Rpl27) transcript as input control. Data were presented as the mean ± SD as relative values compared to the value generated using an amiR-free control vector (n = 3) (see Additional file 2 for individual data values and statistics). d Copy numbers of the transgenes in the liver of Fah−/− mice following intrahepatic delivery of different transposon vectors. Liver DNA samples were collected from Fah−/− mice at 5 months post-treatment. Samples were tested using a Fah transgene-specific qPCR assay. Results were normalized to measurements of the olfactory receptor 16 (Olfr16) gene as an input control, and values were presented relative to one diploid genome (n = 3) (see Additional file 2 for individual data values and statistics)

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