Fig. 1

Phylogenetic position of the new genus of non-photosynthetic green algae, Leontynka. Non-photosynthetic taxa in Chlamydomonadales are highlighted in violet. a Maximum likelihood phylogenetic tree (IQ-TREE, TIM2+F+I+G4 substitution model) of 18S rRNA gene sequences from Chlamydomonadales and related chlorophytes. Non-parametric bootstrap support values calculated from 100 replicates are shown when ≥ 50; black dots denote full support. Previously demarcated main clades [12] are collapsed, and outgroup taxa are not shown for simplicity. The full version of the tree is provided as Additional file 1: Fig. S1. b Phylogenetic analysis of a concatenated dataset of 24 conserved plastome-encoded proteins (5020 amino acid positions) from Chlamydomonadales, including Leontynka pallida, and the sister order Sphaeropleales (sensu lato; see Fučíková et al., 2019). The tree topology was inferred using PhyloBayes (CAT+GTR substitution model), and branch support values correspond to posterior probability (from PhyloBayes)/maximum likelihood bootstrap analysis (IQ-TREE, LG+C60+F+G4 substitution model, 100 non-parametric bootstrap replicates). Branches representing outgroup taxa (Sphaeropleales) are collapsed. The full version of the tree is provided as Additional file 1: Fig. S10. Black dots represent full support obtained with both methods, asterisks denote bootstrap support values < 50. Polytomella spp. and Polytoma oviforme are not represented in part (b) because of the complete absence of a plastid genome and the lack of plastid genome data, respectively