Fig. 4

Evolution of cell-to-cell gene expression variability during hematopoiesis in elderly subjects and SF3B1-mutated MDS. A For each differentiation pathway, a common pseudotime was calculated on the integrated gene cell matrix of the 4 samples. A sub-sampling was performed to have the same number of cells in each cell type per sample. The average intercellular entropy of all genes was then calculated individually for each patient on a sliding window of 50 cells advancing with a step of 10 cells on the common pseudotime. B Intercellular entropy of all genes was calculated on a subsample of 700 HSCs of healthy elderly patients and SF3B1-mutated MDS. A Wilcoxon assay was used to compare the mean intercellular entropy between samples. This was repeated 100 times. Shown is the number of times the resulting test gave a certain level of p-value: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. In 100% of the subsamples, the difference in the mean intercellular entropy between control and MDS patients was very highly significant (p < 0.0001)