Fig. 3

FSS increases activation by CD3/CD28 coated beads. A Graph of increased ZAP70 phosphorylation in Jurkat cells treated with FSS and CD3/CD28 antibody beads for 1 h compared to CD3/CD28 beads alone and untreated cells (N=3). B Graph of NF-κB phosphorylation for Jurkat cells activated with or without FSS, and with or without beads coated with CD3/CD28 for 1 h (N=3). C Graph of cFOS phosphorylation after Jurkat cells were treated with combinations of CD3/CD28 coated beads and FSS for 1 h (N=3). D Cytokine expression 24 h following 1 h of FSS treatment in combination with CD3/CD28 coated beads (N=3). Unpaired t tests were used to measure statistical significance between treatment groups. * p<0.05, ** p<0.01, *** p<0.005, **** p<0.001. Error bars are SD