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Fig. 2 | BMC Biology

Fig. 2

From: GATD3A, a mitochondrial deglycase with evolutionary origins from gammaproteobacteria, restricts the formation of advanced glycation end products

Fig. 2

GATD3A is localized in the mitochondrial matrix. A A schematic of GATD3AWT and GATD3AΔ1-33 expression constructs with FLAG-tag. Immunoblot analysis revealed two bands for GATD3AWT, the larger being GATD3A with its mitochondrial localization signal (MLS) uncleaved. GATase (glutamine amidotransferase), (scale = 10 μm). B Subcellular fractionation reveals absence of GATD3A from a post-mitochondrial cytosolic fraction. C GATD3A in heart mitochondrial membrane, intermembrane and matrix fractions. OM/IM, outer/inner membrane; IMS, intermembrane space. D Fluorescence intensity profile across a mitochondrion using stimulated emission depletion (STED) super-resolution microscopy. GATD3A (green) colocalizes specifically with the mitochondrial matrix protein mtHSP70 (magenta), and not the outer mitochondrial membrane import protein TOMM20 (magenta) (scale = 1 μm)

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