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Fig. 3. | BMC Biology

Fig. 3.

From: The repertoire of testicular extracellular vesicle cargoes and their involvement in inter-compartmental communication associated with spermatogenesis

Fig. 3.

Testicular extracellular vesicles carry miRNA cargoes with differential resistance to RNase in native structure. A Diagram showing the distribution of different small RNA species identified in testicular EVs by RNA sequencing and their corresponding percentage counts. Solid bar represents the subclass. Real-time PCR results showing the level of novel Mir1, Let-7b (mature miRNA), and miR-34a (hairpin) in B testicular EV samples (n=6) and C sperm (n=3) without treatment (untreated), with proteinase K and RNase A treatment (Pk/Ra) or with Pk/Ra treatment in the presence of 1% TritonX-100 (T). Data is presented as mean ± S.D. **P <0.01, ****P<0.0001, ns—not significant. Supporting data values available in Additional file 6: Table S5

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