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Fig. 4. | BMC Biology

Fig. 4.

From: The repertoire of testicular extracellular vesicle cargoes and their involvement in inter-compartmental communication associated with spermatogenesis

Fig. 4.

Inhibition of testicular extracellular vesicles elevated apoptosis of germ cells in mice. A Representative images of TUNEL assay performed on testes sections treated with GW4869 or vehicle (DMSO) in the seminiferous tubule compartment (upper panel) or interstitium compartment (bottom panels). Single injection (T1) or repeated injections (T3) were performed, and samples were collected 2 days (D2) (n=3) or 7 days (D7) (n=5) after injection. Arrowheads indicate TUNEL-positive cells, and the dashed lines indicate tubules with TUNEL-positive cells. B Quantification from these images is shown. C Analysis of proportion of seminiferous tubules with TUNEL-positive cells in different stages of seminiferous tubules. D Concentration of testicular EVs isolated from mice treated with GW4869 or vehicle (DMSO) measured by nanoparticle tracking analysis (T3D7). E Representative Western blot analysis of EV markers CD63 and CD 81 in equal number of testicular EVs from GW4869 or vehicle (DMSO)-treated mice (T3D7). Quantifications are shown in the bottom panel. Data is presented as mean ± S.D. *P <0.05. Supporting data values available in Additional file 6: Table S5. Uncropped blots available in Additional file 7: Fig S8

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