Fig. 3

Conservation of DNA Methylation Valleys (DMVs) across vertebrates. a Bar graphs showing the number of identified PMDs, UMRs and DMVs (UMR >= 5kb) and the percentage of the genome covered by each feature in dermal fibroblasts of the seven studied species. b Gene ontology analysis of genes located in DMVs. The graph shows the enrichments of ontology terms 'regulation of transcription' and 'developmental process' in DMV-associated genes compared to all genes (p value: hypergeometric test). c Genome browser snapshots of WGBS methylation scores showing a conserved DMV overlapping the MEIS1 gene in dermal fibroblasts of the seven species. Each WGBS track shows the percent methylation of individual CpGs between 0 and 100%. CpG islands (green rectangles) and Ensembl gene annotations are shown below the tracks. d Analysis of the overlap of genes located in DMVs in fibroblasts across the seven vertebrate species. Each square in the heatmap represents the percentage of common genes associated with DMVs between two species. Details about the calculation are provided in the Methods section