Fig. 4

Prediction of imprinted DMRs in mammals using WGBS data. a Description of the pipeline used to detect potential imprinted DMRs using WGBS data. To differentiate allelic from partial random methylation, we use single read methylation scores to identify regions that contain a mixture of fully methylated and fully unmethylated reads. We applied a stringent mode to identify regions larger than 350 bp with a minimum of 20 CpGs, and a lenient mode to identify regions with a minimum of 10 CpGs. b Top ranked genes associated with predicted allelic DMRs in fibroblast in stringent and lenient mode in at least 2 species. Asterisks indicate genes previously known to be imprinted in human or mouse. c Genome browser snapshots of WGBS profiles over the PLAGL1 (left panel) and KBTBD6 (right panel) genes in dermal fibroblasts of 6 mammalian species. KBTBD6 is not shown in the rabbit because of lack of gene annotation. CpG islands (green rectangles), predicted DMRs (purple rectangles) and Ensembl or Refseq gene annotations are shown below the tracks