Fig. 6

Aripiprazole mediates DR-like lifespan extension through the AAK-2-DAF-16 pathway. A Survival curves of the daf-16 mutant treated with 100 μM of aripiprazole (Ari) or DMSO at 20 °C (comparison between DMSO and aripiprazole (Ari) treatment: ns = not significant with P > 0.05, log-rank test). B The representative images of worms having cytosolic, intermediate, and nuclear DAF-16 localization in TJ356 transgenic strains. Scale bar = 50 μm. C The percentile of TJ356 animals treated with 100 μM aripiprazole (Ari) or DMSO displaying cytosolic, intermediate, or nuclear localization. D–J Survival curves of mutant daf-2 (D), age-1 (E), akt-1 (F), akt-2 (G), sir-2.1 (H), aak-2 (I), par-4 (J) treated with 100 μM of aripiprazole (Ari) or DMSO at 20 °C (comparison between DMSO and aripiprazole (Ari) treatment: ns = not significant with P > 0.05. ** indicated p < 0.01. ****, p < 0.0001, log-rank test) (see Additional file 2 for supporting data). Experiments were performed in three independent biological replicates