Fig. 6
\(\dot{\varepsilon}\)-S [Ca2+]c flux is dependent on the mechanically gated calcium channel Piezo1. A Ca2+ sampling along T14 in θ = 80° stenosis geometry following treatment of reconstituted blood (10 min) with, DMSO − vehicle control (N = 13 experiments); GsMTX-4 (2.5 μM) [N = 6 experiments] and Ca2+ sampling along T14 in θ = 20° stenosis geometry following treatment of reconstituted blood (10 min) with, DMSO (N = 10 experiments); Yoda1 (25 μM) [N = 9 experiments]. B Frequency histogram showing the distribution of platelet [Ca2+]c (10 nM bins) within Hyperbolic flow at Q = 600 μL/min: DMSO (0.1%v/v); and GsMTx-4 (2.5 μM) and Q = 12.5 μL/min DMSO (0.1%v/v); and Yoda1 (25 μM) [N = 3 experiments]. C Platelet aggregation at stenosis apex θe = 80° following treatment of DiOC6 labelled human whole blood with, DMSO (N = 4 experiments); GsMTX-4 (1.25 μM) [N = 4 experiments]; GsMTX-4 (2.5 μM) [N = 4 experiments]; GsMTX-4 5 μM [N = 4 experiments]. Curves shown are [Agonist] vs. response Variable slope (four parameters) least squares fit + 95% CI. D Platelet aggregation at stenosis apex θ = 20° and 80° following treatment of DiOC6-labelled human whole blood with DMSO (N = 4 experiments); Yoda1 (25 μM) [N = 3 experiments]. Curves shown are [Agonist] vs. response Variable slope (four parameters) least squares fit + 95% CI