Fig. 7
\(\dot{\varepsilon}\)-S [Ca2+]c flux is dependent on Panx1 and P2X1. A Ca2+ sampling along T14 in θ = 80° stenosis geometry following treatment of reconstituted blood (10 min) with DMSO (N = 16 experiments); Apyrase (2 U/mL ≥ 85% ATPase activity) [N = 3 experiments]; NF449 (1 μM) [N = 4 experiments]; Carbenoxolone (10 μM) [N = 3 experiments]. B Frequency histogram showing the distribution of platelet [Ca2+]c (10 nM bins) within Hyperbolic flow at Q = 600 μL/min: DMSO (0.1%v/v); NF449 (1 μM); Carbenoxolone (Cbx; 10 μM) [N = 3 independent experiments]. C Ca2+ sampling along T14 in θ = 20° stenosis geometry following treatment of reconstituted blood (10 min) with DMSO (N = 10 experiments); Yoda1 (25 μM) [N = 9 experiments]; Yoda1 (25 μM) + Cbx (10 μM) [N = 3 experiments]; Yoda1 (25 μM) + NF449 (1 μM) [N = 4 experiments]. D Frequency histogram showing the distribution of platelet [Ca2+]c (10 nM bins) within Hyperbolic flow at Q = 600 μL/min