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Fig. 2 | BMC Biology

Fig. 2

From: A method for ultrafast tissue clearing that preserves fluorescence for multimodal and longitudinal brain imaging

Fig. 2

Fluorescence preservation after clearing. A Rapid clearing of 300-μm-thick slices with CUBIC-1, ScaleSQ(5), FOCM, and AKS. Representative of n = 3 slices (scale bar: 1 mm). B Preservation of fluorescent proteins under different reagent treatments. Representative of n = 3 slices each conditioning (scale bar: 100 μm). C Comparison of the normalised fluorescence of AKS-treated tissue (n = 3) with CUBIC-1 (n = 3), ScaleSQ(5) (n = 3), and FOCM-treated tissue (n = 3); the imaging parameters were the same and images were taken at different time periods. All values are presented as mean ± SEM and included in Additional file 11: Table S3. Statistical significance (*p < 0.05, **p < 0.01, ***p <0.001, ****p < 0.0001) was assessed by a one-way ANOVA followed by Bonferroni’s multiple comparison tests. D Preservation of fluorescent dyes after 10-min AKS treatment. Representative of n = 3 slices each conditioning (scale bar: 100 μm)

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