Fig. 4

Nerve fibre tracking with AKS-cleared Thy1-YFP mice. A–C 3D imaging of 150-μm-thick slices from Thy1-YFP mice before and after AKS treatment. A 3D render of YFP imaged before AKS treatment. B 3D render of YFP imaged after AKS treatment. C XY plane of YFP signal at different image depths before and after AKS treatment. Representative of n = 3 slices each conditioning (scale bar: C, 100 μm). D–F 3D imaging of neurons and nerve fibres in AKS-cleared cortex. Representative of n = 3 slices (scale bars: D, 150 μm; E and F, 50 μm). G Long-range tracing of a single nerve fibre (scale bars: 100 μm). H Imaging of nerve fibres in the corpus callosum and striatum. Representative of n = 3 slices (scale bars: left, 100 μm; right, 100 μm)