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Fig. 7 | BMC Biology

Fig. 7

From: A method for ultrafast tissue clearing that preserves fluorescence for multimodal and longitudinal brain imaging

Fig. 7

AKS treatment for clearing and imaging of the human brain samples. A Transparency of samples (300-μm-thick slice from formalin fixation for 2 years) before and after 10 min of AKS treatment for clearing. Representative of n = 3 slices (scale bar: 2 mm). B Sample size change before and after the AKS treatment. The slice is from fresh-frozen AD sample and immunofluorescence stained with GFAP. Representative of n = 3 slices (scale bar: 2 mm). CG 3D imaging of GFAP-stained tissue before and after AKS clearing at the same imaging position and parameters. Representative of n = 3 slices. C 3D reconstruction of GFAP-stained tissue before and after AKS clearing (scale bar: 40 μm). D Selected single images at the depth of 15 μm, 60 μm, and 110 μm before and after the AKS clearing (scale bar: 15 μm). E Side view of the overlaid 3D-reconstructed GFAP-stained images before and after AKS clearing; note that more astrocytes are visible after clearing (scale bars: 30 μm). F Top view of the overlaid 3D-reconstructed GFAP-stained images before and after AKS clearing (scale bars: 30 μm). G A single image selected from F, showing the overlay of GFAP-labelled signal before and after AKS clearing (scale bars: 30 μm). H High-resolution 3D imaging and reconstruction of astrocytes and neuritic plaques. Representative of n = 3 slices (scale bars: 20 μm for maximum intensity projection (MIP) imaging and 30 μm for XZ plane imaging). I Selected single images at different imaging depths from H, showing the astrocyte fibres inside the neuritic plaques (scale bars: 5 μm)

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