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Table 1 Comparison of AKS properties with other tissue-clearing methods. The table summarises the general properties of AKS and other major published clearing methods. In the column of “handling”, “Needs care” indicates that toxic reagents are used and need to be handled carefully

From: A method for ultrafast tissue clearing that preserves fluorescence for multimodal and longitudinal brain imaging

  Hydrogel embedding Lipid remove Step of operation Fluorescent protein preservation Human brain tissue compatible Handling Normal operating time Reference
ASK No No One step Good, with strong YFP enhancement Yes Very easy Minutes to hours  
CUBIC No Yes One step or multistep Good Yes Easy Hours to days or weeks [9, 44]
ScaleS No No One step or multistep Good Yes Easy Hours to days or weeks [10]
SeeDB No No Multistep Good Not test Easy Several days [13]
FOCM No No One step Good or major loss Not test Very easy Minutes to hours [15]
MACS No No Multistep Good Not test Easy Hour to days [11]
BABB No Yes Multistep Good or major loss Not test Needs care Hour to days [19]
3DISCO No Yes Multistep Major loss Not test Needs care Hours to days [18, 20]
PEGASOS No Yes Multistep Good Yes Easy Hours to days [6]
CLARITY Yes Yes Multistep Good Yes Needs care Weeks [7]
SWITCH Yes Yes Multistep Major loss Yes Needs care Weeks [45]
PACT Yes Yes Multistep Moderate loss Yes Needs care Weeks [8, 10]