A method for ultrafast tissue clearing that preserves fluorescence for multimodal and longitudinal brain imaging

Table 1 Comparison of AKS properties with other tissue-clearing methods. The table summarises the general properties of AKS and other major published clearing methods. In the column of “handling”, “Needs care” indicates that toxic reagents are used and need to be handled carefully

	Hydrogel embedding	Lipid remove	Step of operation	Fluorescent protein preservation	Human brain tissue compatible	Handling	Normal operating time	Reference
ASK	No	No	One step	Good, with strong YFP enhancement	Yes	Very easy	Minutes to hours
CUBIC	No	Yes	One step or multistep	Good	Yes	Easy	Hours to days or weeks	[9, 44]
ScaleS	No	No	One step or multistep	Good	Yes	Easy	Hours to days or weeks	[10]
SeeDB	No	No	Multistep	Good	Not test	Easy	Several days	[13]
FOCM	No	No	One step	Good or major loss	Not test	Very easy	Minutes to hours	[15]
MACS	No	No	Multistep	Good	Not test	Easy	Hour to days	[11]
BABB	No	Yes	Multistep	Good or major loss	Not test	Needs care	Hour to days	[19]
3DISCO	No	Yes	Multistep	Major loss	Not test	Needs care	Hours to days	[18, 20]
PEGASOS	No	Yes	Multistep	Good	Yes	Easy	Hours to days	[6]
CLARITY	Yes	Yes	Multistep	Good	Yes	Needs care	Weeks	[7]
SWITCH	Yes	Yes	Multistep	Major loss	Yes	Needs care	Weeks	[45]
PACT	Yes	Yes	Multistep	Moderate loss	Yes	Needs care	Weeks	[8, 10]

ISSN: 1741-7007