Fig. 5

Changes in transcript packaging are negatively correlated with changes in cellular abundance. A Log2 fold change in VEGF-treated cells vs. untreated cells and in EVs derived from VEGF-treated cells vs. EVs derived from untreated cells for the most increased and decreased protein-coding and lncRNA genes significantly changed in cells. Error bars represent standard error of log2 fold change. * = adjusted p-value < 0.1, NS = adjusted p-value > 0.1. Individual values can be found in Additional file 17. B Log2 change in packaging for the most increased and decreased protein-coding and lncRNA genes in cells. Individual values can be found in Additional file 17. C Log2 change in EV packaging and log2 fold change in cell abundance for protein-coding genes upon VEGF treatment. Orange = adjusted p-value < 0.1 in VEGF-treated vs. untreated cells. D Same as C for long noncoding genes. E Distribution of log2 fold differences of protein-coding and lncRNA genes (combined) between EVs and cells in genes increased in cells by VEGF treatment (top) or decreased in cells by VEGF treatment (bottom). Light purple = log2 fold difference distribution in untreated cells. Dark purple = log2 fold difference distribution in VEGF-treated cells. All analyses were performed using 3 EV and 3 cell samples