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Fig. 7 | BMC Biology

Fig. 7

From: Sorting and packaging of RNA into extracellular vesicles shape intracellular transcript levels

Fig. 7

Inhibiting EV secretion or RNA packaging affects cellular transcript levels. A Distributions of RNA-Seq log2 fold changes of genes enriched in EVs (green) and genes not enriched in EVs (purple) in cells treated with GW4869. N = 3. B Enrichment in EVs vs. cells by RNA-Seq (green scale; n = 3) and fold changes by qRT-PCR of selected genes in cells after treatment with GW4869, Src Inhibitor 1, or Ketoconazole compared to control cells treated with DMSO. Results are means from n=9, 4, and 5 independent experiments for GW4869, Src Inhibitor 1, and Ketoconazole, respectively. Individual values can be found in Additional file 17. C Enrichment in EVs vs. cells by RNA-Seq (green scale; n = 3) and fold changes by qRT-PCR of selected genes after treatment with two siRNA against HNRNPA2B1 or HNRNPA1 compared to cells treated with control siRNA. Results are means from 6, 5, and 4 experiments for siHNRNPA2B1#1, siHNRNPA2B1#2, and siHNRNPA1, respectively. Individual values can be found in Additional file 17. D Model for regulation of intracellular mRNA and lncRNA levels by packaging into EV: increased packaging of mRNA and lncRNA RNA transcripts into EVs leads to decreased abundance of those transcripts in the cell. Decreased packaging of mRNA and lncRNA transcripts into EVs leads to increased abundance of those transcripts in the cell

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