Fig. 3
Metabolome analysis of wild-type and pykA mutants in MC. A, B PCA scores plots (first two components) in the positive (A) and negative (B) ionization mode. Ellipses are the 95% confidence regions. Indicated are the relative activities of PykA. Data correspond to 4 independent extractions (liquid cultures). C Comparison of PykA substrate and product contents in pykAT>D and wild-type (WT) cells. D Comparison of the contents of compounds annotated as pseudaminic acid (Pse) and CMP-pseudaminic acid (CMP-Pse) in pykAT>D and wild-type cells. Data in C and D correspond to 3 independent extractions (solid cultures).*, p > 0.05 ; **, p < 0.01 (Welch’s T-test). Values in bold panels C and D indicate the fold change for each metabolite (wild-type versus pykAT>D)