Fig. 8

PykA directly inhibits the helicase activity of the helicase DnaC and via the primase DnaG. A Time courses (5, 10, 15, 25, and 30 min) showing the helicase activity of DnaC/DnaI in the presence or absence of PykA, as indicated. B Time courses (5, 10, 15, 25, and 30 min) showing the helicase activity of DnaC/DnaI in the presence or absence of PykA and/or DnaG, as indicated. The reactions were carried out as described in “Methods”. Representative native PAGE gels (from two independent experiments) are shown with lanes a and b representing control annealed and fully displaced (boiled) control DNA substrates. Data were plotted as a percentage of displaced primer versus time using GrapPad Prism 4 software. Error bars show the standard deviation from two independent repeat experiments