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Fig. 5 | BMC Biology

Fig. 5

From: Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Fig. 5

Heterologous expression of the fatty acyl reductase candidate gene CsupFAR2 and the desaturase gene CsupYPAQ in Saccharomyces cerevisiae INVSc strain. CsupFAR2 is constructed in the galactose inducible vector pYES52 and CsupYPAQ-CsupFAR2 is constructed in the Cu2+ inducible vector pYEX-CHT. GC/MS analysis of fatty alcohol profiles of yeast harboring the a pYEX52 empty vector supplemented with Z11-16:Me, b pYES52 empty vector without supplement, c pYEX-CHT empty vector supplemented with Z11-16:Me, d pYEX-CHT empty vector without supplement, e CsupFAR2 in pYES52 supplemented with Z11-16:Me, f CsupFAR2 in pYES52 without supplement, g CsupYPAQ-CsupFAR2, and h CsupYPAQ in pYEX-CHT without supplement. Fatty alcohols produced from the introduced genes are shown in red and italics. Displayed chromatograms are representative examples of at least six replicates.

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BMC Biology

ISSN: 1741-7007

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