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Fig. 8 | BMC Biology

Fig. 8

From: Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Fig. 8

Rapid assembly of pheromone biosynthetic pathway in Nicotiana benthamiana for the production and release of moth sex pheromone components. The Cauliflower mosaic virus 35S promoter (p35S) and Octopine Synthase gene terminator (tOCS) have been used to regulate gene expression in plants. ATF1 has also been controlled by trichome-specific promoter pCYP71D16. a Step-wise metabolic engineering strategy for leaf-based pheromone production of (Z)-11-hexadecenol, (Z)-11-hexadecenal, and (Z)-11-hexadecenyl acetate. b Solid-phase microextraction (SPME) of headspace volatiles from a genetically modified N. benthamiana leaf 4 days after infiltration. c GC-MS chromatograms (INNOWax column) of volatiles collected during 24 h from an infiltrated N. benthamiana leaf. d The amount of collected pheromone compounds from released volatiles. e The amount of pheromone compounds from leaf extracts. The error bars represent the standard deviation (SD). N=6. Bars with the same letters represent treatments that are not significantly different at the P=0.05 level (two-way ANOVA followed by t-tests)

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