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Fig. 2 | BMC Biology

Fig. 2

From: Thyroid and endostyle development in cyclostomes provides new insights into the evolutionary history of vertebrates

Fig. 2

Development of the thyroid gland in the Eptatretus burgeri embryo. Dorsal (A, D) and lateral views (G) of fixed embryos immersed in methyl benzoate prior to paraffin embedding. B, B’, E, E’, H, H’ Midline sagittal sections of the hagfish thyroid. B, B’ Sections were stained using hematoxylin and eosin. E, E’, H, H’ Sections including thyroid primordia were stained via in situ hybridization of Tbx1/10A (E, E’) and Lhx3/4A (H, H’) with nuclear fast red in a previous study [17]. The area surrounded by dashed lines indicates thyroid primordium. Lateral (C, F, I) and frontal (C’, F’, I’) views of 3D reconstructed images. The ectoderm and endoderm are colored light blue and yellow, respectively. Dashed lines indicate the boundary between stomodeum and pharynx. Thyroid is colored red. Both cardiac field and blood vessel are transparently colored light red. pp, pharyngeal pouch; ect, ectoderm; nc, notochord; ot, otic vesicle; end, endoderm; heart, ht; ventral aorta, va; bs, bifurcation site of ventral aorta. See Fig. 1 for other abbreviations. Bars, 1 mm (B, E, H) and 200 μm (B’, E’, H’)

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