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Fig. 8 | BMC Biology

Fig. 8

From: Irisin mediates beiging of adipose-derived mesenchymal stem cells through binding to TRPC3

Fig. 8

Trpc3 mediates IRISIN-induced thermogenesis in vivo. A-C Different parts of adipose tissues obtained from a normal C57BL/c wild group, IRISIN-treated C57BL/c wild group, combination with IRISIN and TRPC3 inhibitor (PYR3)-treated C57BL/c wild group, and IRISIN-treated Trpc3/ knockout C57BL/c group were lysed to detect the protein levels of UCP1, p-ERK, and p-AKT by western blot. D Adipose tissues obtained from different parts were lysed to detect the protein levels of UCP1 using immunohistochemistry. E Mesenchymal stem cells (MSCs) were isolated from the adipose tissue of wild-type mice, Trpc3 heterozygous mice, and Trpc3/ knockout mice. IRISIN was used to stimulate these MSCs. Cells were collected from the three groups of mice at 0, 6, 12, and 24 h after stimulation. Western blotting was used to detect the expression of UCP1 proteins. F–H Microdialysis method was used to detect the metabolites such as glycerin, epinephrine, and norepinephrine in adipose dialysate of C57BL/c wild group, IRISIN-treated C57BL/c wild group and IRISIN-treated Trpc3/ knockout C57BL/c group

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