Fig. 1
Fetal E3 exposure improves reproduction in female offspring. Eight-week-old pregnant female CD-1 mice were treated with vehicle DMSO (CT) or E3 (E3). At 8 weeks after birth, the female offspring were bred to untreated male CD-1 mice. A The percentage of pregnancy occurrence (vaginal plug, pregnancy, resorption, and stillbirth), litter size (number of pups), and percentage of deliveries (premature labor, term labor, and post-term labor). Error bars: mean + SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001 by Student t test, N = 55 CT, 40 E3-treated female offspring. B The uterine morphology of control (CT) and E3 female offspring that had received treatment as a fetus. C Representative micrographs of hematoxylin and eosin staining of the uteri. The images are representative of 10 mice