Fig. 3
Prenatal exposure of E3 enhances expression of epigenetic modifiers in female offspring and enhances ER binding to epigenetic modifiers. A Representative immunoblots of epigenetic modifying proteins from the uteri of prenatally vehicle- (CT, n = 3 mice) and E3-treated mice (E3, n = 3 mice). B Increased histone methylation in prenatally E3-treated mice (n = 2 mice) compared to those treated with vehicle control (n = 2 mice). C Co-immunoprecipitation (IP) of ERα/β with DNA/histone modifiers from Ishkawa cells expressing Flag (Vec), Flag-ERα, or Flag-ERβ respectively, using an anti-Flag antibody followed by immunoblotting (IB) using the indicated antibodies. D Co-IP of Flag-ERα with DNA/histone modifiers from Ishkawa cells treated with DMSO (CT), E2 (E2), E3 (E3), bisphenol A (BPA), or diethylstilbestrol (DES). The immunoblots show the proteins bound to Flag-ERα and are representative of 3 independent experiments. Uncropped blots available in Additional file 3: Fig. S7