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Fig. 7 | BMC Biology

Fig. 7

From: The steroid hormone estriol (E3) regulates epigenetic programming of fetal mouse brain and reproductive tract

Fig. 7

Prenatal exposure to E3 altered the effect of DRD2 agonist sumanirole (2 μM) on membrane potential (MP) in layer 2/3 pyramidal cells and c-Fos expression in the prefrontal cortex. A–C Perforated and whole-cell patch clamp recording. A Time courses showing the effect of sumanirole on MP in representative pyramidal cells from control (CT, top trace) and E3-treated (E3, bottom trace) mice. X-axis unit = minute; Y-axis unit = mV. The entire time course includes baseline (aCFS+TTX), sumanirole application and washout of sumanirole. B Two sample whole-cell recording traces. C Pooled results showed that MP was hyperpolarized significantly in control mice (blue bars) but not E3-treated mice (red bars). * P < 0.05, one-way ANOVA; ns P > 0.05, one-way ANOVA). D Immunofluorescence staining for c-Fos in the frontal cortex of the E3-treated and vehicle (DMSO) control group (CT). c-Fos expression in the front cortex is upregulated in the E3-treated female offspring. E The c-Fos fluorescence intensity per cell acquired by using image J. F The number of c-Fos-positive cells per 0.05 mm2. Error bars: mean + SD. ***, p < 0.001 by Student t test. N = 13/group

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