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Fig. 8 | BMC Biology

Fig. 8

From: The steroid hormone estriol (E3) regulates epigenetic programming of fetal mouse brain and reproductive tract

Fig. 8

E3 treatment impacts formation of ER-epigenetic modifiers complex and their binding to target genes in neurons. Neuroblastoma SH-SY5Y cells were transfected with the Flag chimera expressing plasmids (Vec), Flag-ERα, or Flag-ERβ respectively, and then treated with/without E3. A Co-immunoprecipitation (IP) of ERα/β and DNA/histone modifiers with an anti-Flag antibody followed by immunoblotting (IB) using the indicated antibodies. The input indicates the expression level in the whole cell lysate before IP. B, C Neuroblastoma SH-SY5Y cells were treated with/without E3 (CT, E3), and B ChIP (chromatin immunoprecipitation) was performed using an anti-estrogen receptor α/β (ESR1/ESR2) antibody (First ChIP) and then with an anti-SUZ12 or DNMT1 antibody (Re-ChIP). The bound DNA was then identified by sequencing. The chart shows the numbers of genes involved in the nervous system development and function. C, D Validation of the target DNA sequences from B by Chip-PCR. The data are representative of three independent experiments. Uncropped blots available in Additional file 3: Fig. S7

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