Fig. 2

Generation of AsCas12a variants with increased efficiency by introducing the high-activity substitution. a The mutation sites of the high active AsCas12a variants. b Tag-seq-based comparative analyses of wild-type AsCas12a (WT), AsCas12a variant RKA (RKA), AsCas12a variant RKK (RKK), and AsCas12a-HF (HF, the reported high-fidelity variant of enAsCas12a) with twenty-two sgRNAs targeting twelve genes (also see Additional file 2: Figure S3). c Normalization of on-target activity of RKA, RKK, and HF to wild-type AsCas12a. d Total number of off-target sites detected with the twenty-two sgRNAs. e Specificity index (value was calculated by the ratio of total on-target reads to the on-target reads plus the off-target reads within the twenty-two sites). f Detection of the editing abilities for the non-canonical PAM with AsCas12a-RKA. Mean values are presented with SEM, n=4 independent experiments. Indel was revealed by Deep-seq