Fig. 3
From: Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity
Multiplex editing with the AsCas12a variants. a Schematic of the multiplex editing. b Tag-seq-based comparative analyses of wild-type AsCas12a (WT), AsCas12a variants KA, KK, RKA, RKK, and HF with a single crRNA array targeting six sites. c Total number of off-target sites detected with the six sgRNAs within a single crRNA array. d Specificity index (value was calculated by the ratio of total on-target reads to the on-target reads plus the off-target reads counts within these six sites). e Normalization of on-target activity of KA, KK, RKA, RKK, and HF to wild-type AsCas12a in multiplex editing