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Fig. 6 | BMC Biology

Fig. 6

From: Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity

Fig. 6

Generation of high-performance LbCas12a variants. a The conserved sites in AsCas12a and LbCas12a, underlined with red. b The mutation sites of LbCas12a variants. c Tag-seq-based comparative analyses of wild-type LbCas12a (WT), LbCas12a variant KA (KA), LbCas12a variant KK (KK), LbCas12a variant RKA (RKA), and LbCas12a variant RKK (RKK) with fifteen sgRNAs targeting nine genes. d Total number of off-target sites detected with the fifteen sgRNAs. e Specificity index (value was calculated by the ratio of total on-target reads to the on-target reads plus the off-target reads within the fifteen sites). f Normalization of on-target activity of LbCas12a-KA, LbCas12a-KK, LbCas12a-RKA, and LbCas12a-RKK to wild-type LbCas12a

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