Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: TrackUSF, a novel tool for automated ultrasonic vocalization analysis, reveals modified calls in a rat model of autism

Fig. 4

Analyzing rat social vocalizations using TrackUSF. A An example of a spectrogram showing a sequence of USVs produced during male-female interactions of SD rats and their detection by USVSEG (purple boxes above). USFs detected by TrackUSF (threshold = 1 a.u.) are marked by the orange (cluster 9) and green (cluster 10) asterisks below. B A comparison of the mean percentage of manually detected USV which were automatically detected by either TrackUSF or USVSEG, from 6 audio clips of 10-min SD rat male-female interaction. C Percentage of all automatically detected USVs that were found to represent non-USV signals (false-positive detections) for either TrackUSF or USVSEG. Each point represents a distinct audio clip. D Number of detected USFs for each audio clip plotted as a function of the number of the corresponding manually detected USVs. Each dot represents a distinct audio clip. E Mean PSD profiles of either USVs detected manually (yellow), by USVSEG (pink) or USFs detected by TrackUSF (green). The PSD analysis of TrackUSF detected USFs was conducted after scaling the PSD curve of each cluster to the number of USFs and summing the curves. F The t-SNE analysis of all USFs extracted from audio recordings of 45 sessions of free interactions and 45 sessions of SP test, conducted with male SD rats (male-male vocalizations). Each USF is represented by a dot, color-coded for the experimental setting. G DBSCAN automatic clustering of the USFs based on the t-SNE analysis result. H Examples of spectrograms showing signals composed USFs from three distinct clusters, including noise (cluster 1) or vocalization (clusters 5 and 9) signals. Each spectrogram contains USFs of a single cluster only. I Mean PSD profiles of USFs from clusters 1–4 (noise, blue), 5–8 (aversive calls, orange), and 9 (appetitive calls, purple). J Pie-charts of the various sessions categorized according to the number of USFs per session, separately for appetitive (cluster 9, upper charts) and aversive (clusters 5–8, lower charts) USFs recorded during SP test (left charts) and free interaction (right charts) sessions

Back to article page