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Fig. 7 | BMC Biology

Fig. 7

From: ASC proneural factors are necessary for chromatin remodeling during neuroectodermal to neuroblast fate transition to ensure the timely initiation of the neural stem cell program

Fig. 7

Proneural bound genomic elements exhibit spatiotemporal enhancer activity and proneural dependency. A Summary of enhancer spatiotemporal expression patterns in wt and Dfsc(1)scB57 (*) embryos. B Embryos expressing the upstream nvy-KV14 reporter. C Embryos expressing the upstream scrt-KV10 reporter. D Embryos expressing the upstream dpn-KV23. E The intronic phyl-KV4 reporterin stage 10 embryos. F The 3′ prime tap-KV21 reporter. G The KV8 reporter proximal to the short brat isoforms H The proximal to TSS insc-KV1 reporter. In the genomic insets, black arrows indicate the extent and cloning orientation of the genomic elements in the lacZ expressing vectors. The > symbol next to gene names shows the orientation of transcription. Overlay is the composite of the three channels. The genomic coordinates and characteristics of proneural bound enhancer regions cloned for the generation of lacz reporter flies provided in Additional file 2: Table S7

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