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Fig. 3 | BMC Biology

Fig. 3

From: Identification of LINE retrotransposons and long non-coding RNAs expressed in the octopus brain

Fig. 3

A full-length potentially active LINE transcribed in Octopus vulgaris. a Domain composition of the discovered LINE (comp36575_c1_seq3). The black line represents the transcript, the black box represents the location of the ORF, and the colored boxes represent the protein domains (Endo, endonuclease; RT, reverse transcriptase; C2H2, zinc finger). The numbers are relative to the nucleotide positions in the transcript. b Schematic alignment highlighting the conserved catalytic amino acids in the group of LINEs adapted from [38] plus the octopus element. The color code of the domains is the same as in a; amino acids critical for EN (*), RT (!), and retrotrasposition (#). c Electrophoresis from RT-PCR of the LINE showing the expression from three different animals. d Phylogenetic tree based on 100 LINEs from [39] (see Additional file 1: Table S4) plus the octopus element in red. e LINE copy number variation analysis using quantitative real-time PCR with Taqman probes. f Expression levels of the LINE based on RNAseq data. g Expression levels of Piwi-like protein 1 (comp33731_c0_seq1) from RNAseq data

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