Fig. 3

Instability of 1Q-WPBs. A Predicted WPB size distribution (stars) compared to real data (blue bars), where 1Q-WPBs display instability, α, greater than longer organelles. B HUVECs were fixed and processed to label the Golgi apparatus with an anti-TGN46 antibody and WPBs and cell boundaries as described in Fig. 1B. The Golgi area was masked to identify newly-made WPBs and separate them from the cytoplasmic organelles (panels on the right); scale bar: 20 μm. C WPB size distributions at the Golgi and in cytoplasm measured after the image segmentation illustrated in B. D WPB size distribution in control (si-Luciferase) and MyRIP-depleted (si-MyRIP) cells