Fig. 7

AalbOpsin1 mediates negative phototaxis behavior of field-collected Ae. albopictus. a, b Photobehavior of field-collected Ae. albopictus. a Photobehavior of female adult (n = 300 females for site A, n = 150 females for site B and site C). b Photobehavior of field-collected larvae and pupae (n = 150 larvae or pupae per group for site A and site C, n = 100 first and third instar larvae for site B, n = 150 pupae for site B). c, d Photobehavior of field-collected Ae. albopictus injected with dsRNA against AalbOpsin1. c Knockdown efficiency of dsAalbOpsin1 in field mosquitoes was measured with qPCR (each dot denotes one mosquito; n = 10 females per group). d Photobehavior of field-collected Ae. albopictus with AalbOpsin1-silenced. Starting from 3 days post-gene silencing, mosquito photobehavior was assessed with Y-maze assay. n in the figure denotes the total number of female mosquitoes tested for each group. a–d Data are presented as mean ± SEM. n denotes the total number of mosquitoes tested. Photobehavior was analyzed using one sample t test or Wilcoxon signed-rank test. c, d Two-way ANOVA, followed by Sidak’s multiple comparisons test was performed for testing among different groups. *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant