Fig. 2

GO biological analysis of UB dataset. A GO biological process analysis of UB RNA-Seq data (n = 4 kidneys/genotype) was carried out with the ToppFun tool (application of ToppGene Suite). The figure shows the genes identified in our dataset in blue (0–140) and those within annotated functions in ToppFun in red (0–2000). B The heatmap analysis of the top four biological processes identified by ToppFun in the UB dataset reveals clear expressional changes, both down and up, between control (green) and MAPK/ERK-deficient UB (Dko, yellow) within the biological processes of cell motility, proliferation, response to stimulus, and epithelium development. Detailed lists of all biological processes and the genes that make them up are provided in Additional file 4: Table S3