Fig. 3
From: Genome of the parasitoid wasp Cotesia chilonis sheds light on amino acid resource exploitation
Rearing of C. chilonis with different rearing media in vitro. A In vitro rearing of C. chilonis. Five days after parasitism, larvae were put on the membrane of a Transwell chamber; then, the Transwell was placed in the well containing 250 μl of C. chilonis rearing medium so that the wasp larvae could reach the nutrients. B Survival rates of C. chilonis larvae developed on 13 different rearing media. Positive control: Grace’s Insect Medium, containing 20 amino acids (n = 30); negative control uses positive control medium minus the ten amino acids that C. chilonis cannot synthesize, ASL-AA, Arg, n = 30; single amino acid deficiencies use control media minus only one amino acid, indicated by “-” superscript, e.g., Gly deficiency (Gly-) indicates excluding glycine only. The Gehan-Breslow-Wilcoxon test was used for survival rate statistical analyses, and the Benjamini-Hochberg method was used for multiple testing correction. The statistical results of pairwise group comparisons are indicated