Fig. 5

Parasitism by C. chilonis suppresses the host’s amino acid biosynthetic pathways within 72 h after parasitism. A The amino acid biosynthetic pathways were modified from Fig. 2. The pathway genes in gray are lost in the genome of C. suppressalis. The pathway genes in green are present in the genome of C. suppressalis. Gene expression changes at 24 h (P1), 48 h (P2), and 72 h (P3) after parasitism were shown in three squares with different colors (gray, no significant expression change; green, significantly downregulated after parasitism, padj < 0.1). Gene set enrichment analyses (GSEA) showed that the genes in the amino acid biosynthetic pathways of C. suppressalis were largely downregulated at 24 h (B), 48 h (C), and 72 h (D) after parasitism. The top portion of the GSEA plot shows the running enrichment score for the gene set as the analysis walks down the ranked list. The middle portion of the GSEA plot reflects the positions of the members of the gene set in the ranked gene list. The bottom portion of the GSEA plot displays the value of the ranking metric along with the list. Three independent biological replicates of each sample for gene expression analyses were conducted (n = 3). Expression source data are included in Additional file 1: Table S8