Fig. 3
From: Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs
Contraction of H3K27me3 domains seen in monocytes and erythroblasts coincides with a loss of LOCKs in progenitors. A Genome browser view of H3K27me3 density on chromosome 20 across cell types as indicated by the color legend (bottom right). The red box indicates H3K27me3 LOCK (FDR <0.05) in progenitor cells that is absent in monocytes and erythroblasts. B Venn diagram of LOCKs in all progenitors (CD34+CD38−; MEP; CMP; GMP) compared to those in monocytes, erythroblasts, B cells, and T cells. C Violin plot of base pairs marked by H3K27me3 within LOCKs across cell types, as indicated (***p <0.001). Maximum values for normalized H3K27me3 densities enriched within LOCKs in erythroblasts (D) and monocytes (E). F Barplot showing the percentage overlap of H3K27me3-enriched regions within progenitors’ LOCKs also in monocytes and erythroblasts with H3K27me3-marked regions identified in H1 ESCs. G Cartoon of contraction of H3K27me3-marked nucleosomes (red) during monocyte/erythroid differentiation. (***p < 0.001)